Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant
2021
Yang Lu | Shuangxia Luo | Qiang Li | Na Li | Wenchao Du | Ping Yu | Xing Wang | Weiwei Zhang | Shuxin Xuan | Xuan Zhou | Jiajia Shen | Jianjun Zhao | Yanhua Wang | Xueping Chen | Shuxing Shen
Dwarfism is a desirable trait in eggplant breeding, as it confers higher lodging resistance and allows simplified management and harvest. However, a few dwarf mutants have been reported, and the molecular mechanism underlying dwarfism in eggplant is completely unknown. Here, we report a dwarf mutant (<i>dwf</i>) isolated from an ethyl methyl sulfonate (EMS)-induced mutant library. The hypocotyl length, plant height, and length of internode cells of <i>dwf</i> were significantly decreased compared to those of the wild-type parent ‘14-345’ (WT). Differential gene expression analysis revealed that GA-related genes, including <i>GA2ox</i> and <i>DELLA,</i> were up-regulated whereas the gibberellin (GA<sub>3</sub>) content decreased in <i>dwf</i>. Moreover, exogenous GA<sub>3</sub> treatment significantly increased the relative growth rate of <i>dwf</i> compared to WT, further indicating the important roles of GA in regulating the dwarf phenotype of <i>dwf</i>. Collectively, our findings shed light on GA-mediated dwarfism in <i>dwf</i> plants and offer a good germplasm that could be used for eggplant dwarfism breeding in the future.
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