The stability of plant nuclear genome is a necessary condition for the faithful transmission of genetic information through cell lineages. When DNA damage occurs due to various impairments, cells start a number of repair processes including ligation of broken chromosomes. As a result, dicentric chromosomes can be formed. Dicentrics are easily detectable as anaphase bridges during following mitosis. Using Arabidopsis as a model plant, we developed a sensitive cytogenetic assay to identify specific chromosomal rearrangements. Here we show Arabidopsis tert-/- and atm-/- mutants and their chromosome rearrangements and fusions analysed by fluorescence in situ hybridization (FISH). The method is based on successive rounds of FISH with chromosome-specific probes and the comparison of resulting FISH images.
显示更多 [+] 显示较少 [-]
AGRIS - 国际农业科技情报系统
Share
出版者
Czech Academy of Agricultural Sciences
其它主题
Rdna loci; Chromosomal fusions; Bac probes
语言
英语
2024-12-12
2026-02-03
DOAJ