Molecular and Biochemical Methods Used for the Identification of Globodera Species in Slovenia (from 6th Conference of European Foundation for Plant Pathology, Prague, Czech Republic, 8-14 September 2002)

S. Širca; G. Urek; V. Meglič

Molecular and Biochemical Methods Used for the Identification of Globodera Species in Slovenia (from 6th Conference of European Foundation for Plant Pathology, Prague, Czech Republic, 8-14 September 2002)

2003

S. Širca | G. Urek | V. Meglič

Globodera species identification is based on specific morphological characteristics which are included in the majority of keys used for Globodera identification. To confirm and make more precise the morphometrical method, other methods have been developed. At Agricultural Institute of Slovenia, molecular and biochemical methods are applied. With the use of PCR-RFLP method we try to distinguish between G. rostochiensis, G. pallida and G. tabacum. Patterns of nematode DNA digested with restriction endonucleases and subjected to agarose gel electrophoresis are analysed. Differences in DNA sequence result in the number and size of fragments produced (RFLPs). For the differentiation of all three nematodes we have introduced a method for protein electrophoresis. Samples are compared after being separated by polyacrilamide slab gel electrophoresis. Proteins are visualised after silver staining.

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