Analysis of genetic diversity among high sucrose genotypes of sugarcane (Saccharum spp.) derived from CoC 671 using sugarcane specific microsatellite markers
2010
D. Leena Lavanya, and G. Hemaprabha
Sugarcane breeders target genetic improvement for sucrose content using conventional and molecular approaches in orderto produce more sugar per unit area. The sucrose rich clone viz. CoC 671 is a widely used parent in juice qualityimprovement programmes. The present study is carried out using twenty eight derivatives of CoC 671 bred throughcrossing as male parent, as female parent, selfing, through somaclonal variation and induced mutagenesis. The cloneswere evaluated based on 30 sugarcane specific STMS markers in order to identify more diverse types to be used asparents and to compare the efficiency of different breeding methods to generate more variability for imparting selection..Genetic diversity was estimated using Dice, Jaccard’s and Simple Matching coefficient methods. STMS markers werefound to be suited to detect genetic differences among the clones of common descent as supported by the lower similarityindex values. Mean genetic similarity among the somaclones (SI value = 0.72), mutants (SI value = 0.81), clones derivedfrom CoC 671 as a female (SI value = 0.73) parent and male parent (SI value = 0.70) clearly showed that the clones withCoC 671 as the male parent were more diverse and indicated the role of male parent in creating a more segregatingpopulation than when used as female parent. Tissue culture based selection through somaclonal variation and inducedmutations were less efficient in creating variability than the conventional methods. More diverse clones and combinationswere identified to be used in hybridization for sucrose improvement in sugarcane.
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