Functional Analysis of Three Rice Chloroplast Transit Peptides
2019
Lei He | Guang Chen | Sen Zhang | Zhennan Qiu | Jiang Hu | Dali Zeng | Guangheng Zhang | Guojun Dong | Zhenyu Gao | Deyong Ren | Lan Shen | Longbiao Guo | Qian Qian | Li Zhu
Chloroplast transit peptides (CTPs) can be used to transport non-chloroplastic proteins into the chloroplasts. Here, we studied the CTPs of three rice (Oryza sativa L.) chloroplast-localized proteins and found that their CTPs could be used to transport non-chloroplast-localized proteins into the chloroplasts. Fusion proteins lacking the CTP remained located in the cytoplasm. Furthermore, we constructed green fluorescent protein fusion vectors with the three CTPs and three non-chloroplast-localized proteins, Ghd10, MULTI-FLORET SPIKELET1 (MFS1), and SHORTENED UPPERMOST INTERNODE 1 (SUI1). After transforming these constructs into rice protoplasts, the fusion proteins all localized in the chloroplasts. Collectively, our results showed that these CTPs can transport non-chloroplast-localized proteins into the chloroplasts, and more importantly, these CTPs can be applied to engineer chloroplast metabolism. Keywords: rice, chloroplast transit peptide, translocated function, subcellular location
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