The effect of photoperiod and temperature on hatching and egg development of Bradyporus latipes (Orthoptera: Tettigoniidae)
2022
Farshad Haghighian | Jalal Jalali Sendi | Yaser Rahimain
Two factors that affect egg diapause in Bradyporus latipes are photoperiod and temperature. In the current study the first instars of insect nymphs collected in fields at Dotu loCated in Shahre-Kord County, Iran. The samples werer reared in laboratory at 15 to 30oC and relative humidity of 40-60 percentages. The bottom of cages was filled with soil and with a 100-watt bulb day-length were controlled. Two photoperiod for adults, 16L:8D and 12L:12D were placed. Insect feeding plants during the breeding period were Phlomis olivierii, Convolvolus sp and Bromus tomentellus to adults emerged and mated in each photoperiod. The eggs were collected from soil weekly. Eggs maintained on moist filtere paper in petrdish and filterpaper moisted every week. For study the egg duration and egg hatching numbers, the eggs that were laid by females, incubated at 15oC, 20oC, 25oC and 30oC in incubators in two photoperiod inlaboratory. The eggs maintained in this situations for 30 weeks and egg hatching number were calculated. 80 eggs at 25oC were transferred to 5oC and 10oC for 30, 60 days in two maternal photoperiods to determine the effect of wintering on insect eggs diapuase and then return to primary situation. The experiments continued to next spring and 2nd next spring. Also 80 eggs from each maternal photoperiod were kept outdoor in field. The duration of egg periods and egg hatching number recorded and analysed by SAS software and the comparison of means were conducted by Tukey's test. The results of current study demonsterated that the maternal photoperiod or photoperiod that effect females in oviposition time and temperature are more important in egg hatching number and egg duration.
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