TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples
2023
Rahman Mohammad Sabri Abdul | Khor Kuan Hua | Khairani-Bejo Siti | Lau Seng Fong | Mazlan Mazlina | Roslan Mohd Azri | Ajat Mohd Mokrish Md | Noor Mohd Akmal Mohd
Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated with kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation and determine the severity of infection, especially in the acute stage, allowing early detection for immediate treatment decisions. To our knowledge, real-time PCR has not been routinely adopted for clinical investigation in Malaysia. This study evaluated TaqMan real-time PCR (qPCR) assays diagnosing leptospirosis and compared their applicability to clinical samples from dogs with kidney and/or liver disease against a conventional PCR reference.
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