Influence of Metisevit Plus feed additive on the activity of the glutathione system of the body of bulls under conditions of man-caused load

Pollution of the environment with heavy metals due to artificial activities of the population has led to several problems for agricultural production. The work aims to study the influence of Metisevit Plus feed additive on the state of the glutathione system of the body of bulls under conditions of man-caused load. The research was conducted based on the private agricultural enterprise “Ukraine” Dubrovytsia district of Rivne region on 12 bulls six months old, Ukrainian black-and-white dairy breed, which was formed into two groups of 6 animals each: 1 group – control, bulls were on a standard diet; Group 2 – experimental, bulls were fed a feed additive “Metisevit plus” at a dose of 0.5 g/kg of feed. According to studies, cadmium and lead load reduce the activity of the glutathione link in the antioxidant defense system of bulls. Feeding the Metisevit Plus feed additive to the bulls of the experimental group for 30 days helped increase the level of non-enzymatic and enzymatic activity of the glutathione system. A probable increase in the level of reduced glutathione was found on 30 and 40 days of the experiment, were compared with the control group of animals, it increased by 7.2 and 7.1 %, respectively. In the study of the activity of glutathione peroxidase and glutathione reductase, it was found that in these study periods they ranged from 35.7 ± 1.61 and 35.3 ± 1.55 nmol NADPH/min per 1 mg of protein (P ≤ 0.01) and 1.67 ± 0.041 and 1.64 ± 0.039 nmol NADPH/min per 1 mg of protein (P ≤ 0.001). Thus, studies on bulls indicate that the feed additive Metisevit Plus, when fed to bulls, under a load of cadmium and lead, contributed to the activation of the glutathione antioxidant defense system by increasing the level of enzymatic and non-enzymatic units. Our studies confirm the feasibility of using a feed additive, Metisevit Plus, to prevent lead-cadmium toxicosis.