Evaluation of direct metagenomics and target enriched approaches for high-throughput sequencing of field rabies viruses

Orłowska Anna; Iwan Ewelina; Smreczak Marcin; Rola Jerzy

Evaluation of direct metagenomics and target enriched approaches for high-throughput sequencing of field rabies viruses

2019

Orłowska Anna | Iwan Ewelina | Smreczak Marcin | Rola Jerzy

High-throughput sequencing (HTS) identifies random viral fragments in environmental samples metagenomically. High reliability gains it broad application in virus evolution, host-virus interaction, and pathogenicity studies. Deep sequencing of field samples with content of host genetic material and bacteria often produces insufficient data for metagenomics and must be preceded by target enrichment. The main goal of the study was the evaluation of HTS for complete genome sequencing of field-case rabies viruses (RABVs).

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