Infections and molecular characterization of anisakid nematodes from two species of marine fish northwest Arabian gulf
2022
Majid A. Bannai | Muna M. Jori
The present study provides new insight into valuable information on the diverse structure of the anisakid population, discusses the limited species richness, and also discusses the relationship with other closely diversity-related taxa in NCBI databases in the <em>Epinephelus diacanthus </em>and<em> Epinephelus coioides</em> fish. The fishing area consists of various locations in the Arabian Gulf. A total of 69 <em>E.coioides </em>and<em> E. diacanthus</em> were examined, (n= 48) were infected. Larval stages (n=1,119). Isolated larvae were encysted within the mesenteries peritoneum and viscera of fish organs, with a prevalence of 81.25% of infection and 59.459 % in the <em>E. diacanthus and E. coioides</em> respectively. Molecular analysis was carried out on thirty individuals of nematode parasites who have examined the morphology and showed some appearance differences, by amplifying internal transcribed spacers ITS and ITS-1 of nuclear rDNA (rDNA) by PCR using the primer sets NC5/NC2 and SS1/NC13R of DNA products. Evolutionary analyses were conducted in MEGA X. based on the identity percentage in the GenBank database showed that they belong to anisakid nematodes, in particular, they belong to nine distinct taxa within the <em>Hysterothylacium</em> spp. The presence of the same species individuals in one host may be the cause of these genetic variations at the species level, and that's what the current study has recorded. It has been found that there is an overlap in the order of nitrogen bases between the same species, and this occurs through the fertilization process, while the rest is clean or have only a few parasites.
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