A Method for the Analysis of African Swine Fever by Viral Metagenomic Sequencing
2021
ChiHai Ji | ChiHai Ji | ChiHai Ji | ChiHai Ji | ChiHai Ji | JingZhe Jiang | YingFang Wei | YingFang Wei | YingFang Wei | YingFang Wei | YingFang Wei | ZhiYuan Wang | ZhiYuan Wang | ZhiYuan Wang | ZhiYuan Wang | YongJie Chen | YongJie Chen | YongJie Chen | YongJie Chen | YongJie Chen | ZhanZhuo Mai | ZhanZhuo Mai | ZhanZhuo Mai | ZhanZhuo Mai | MengKai Cai | MengKai Cai | MengKai Cai | ChenXiao Qin | ChenXiao Qin | ChenXiao Qin | ChenXiao Qin | Yu Cai | Yu Cai | Yu Cai | Yu Cai | HeYou Yi | HeYou Yi | HeYou Yi | HeYou Yi | Guan Liang | Guan Liang | Guan Liang | Guan Liang | Gang Lu | Gang Lu | Lang Gong | Lang Gong | Lang Gong | Lang Gong | GuiHong Zhang | GuiHong Zhang | GuiHong Zhang | GuiHong Zhang | GuiHong Zhang | Heng Wang | Heng Wang | Heng Wang | Heng Wang | Heng Wang
In 2018, there was an outbreak of African swine fever (ASF) in China, which spread to other provinces in the following 3 years and severely damaged China's pig industry. ASF is caused by the African swine fever virus (ASFV). Given that the genome of the African swine fever virus is very complex and whole genome information is currently inadequate, it is important to efficiently obtain virus genome sequences for genomic and epidemiological studies. The prevalent ASFV strains have low genetic variability; therefore, whole genome sequencing analysis provides a basis for the study of ASFV. We provide a method for the efficient sequencing of whole genomes, which requires only a small number of tissues. The database construction method was selected according to the genomic types of ASFV, and the whole ASFV genome was obtained through data filtering, host sequence removal, virus classification, data assembly, virus sequence identification, statistical analysis, gene prediction, and functional analysis. Our proposed method will facilitate ASFV genome sequencing and novel virus discovery.
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