The Differences in Bioactivity between Crude and Purified Cholera Toxin from Local Isolate of Vibrio cholera
2012
Amina N. Al- Thwani1
In this investigate Cholera toxin was purified from the local clinical isolate designated as V. cholerae S . Few steps were employed for purification of CT including concentration of the protein, back extraction, and gel filtration. The bioactivity of CT reflected that crude and purified toxin had lethal activity on Balb/c mice in which a dose of 2.5µg/ ml of purified toxin caused mice death with symptoms such as muscle cramps and tachycardia while erythematous and induration was seen at intradermal I. d injection of skin of Guinea pig. Histopathological examination for liver, intestine, adrenal gland and spleen of white mice showed that liver tissue was more affected compared with spleen, intestine and adrenal gland tissues.
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