Adaptation of locally isolated equine herpesvirus (EHV-1) on different cell lines
2018
Safaa A. Warda
Locally isolated EHV-1 (2002) was successfully adapted to different available cell lines (BHK, VERO, MDBK and MDCK). Characteristic cytopathic effect (CPE) was observed from the first passage and the virus growth became regular with constant incubation periods at 2nd day in BHK and VERO cells 4th day in both MDBK and MDCK cells post inoculation with maximum titers of 7, 8.5, 7.75, 6.2 log10 TCID50/ml in BHK6, VERO10, MDBK5 and MDCK5 cell lines; respectively. Growth kinetics of EHV-1 in VERO cells, showed initial increase in cell associated viruses (CAV) and cell free viruses (CFV) were at 8 and 48 hours post inoculation then reaching their peak at 72 and 96 hours post inoculation in CAV and CFV; respectively. Detection of viral protein in infected cells was done using IFT. It revealed that high fluorescence was shown at 4 till 72 hours post inoculation in CAV and 72 hours in CFV. In conclusion, EHV-1 is more cell associated and IFT is considered as a rapid and sensitive test for detection of viral protein
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