ANTIBIOFILM AND ANTIBACTERIAL ACTIVITY OF PROSOPIS JULIFLORA LEAVES EXTRACT AGAINST ESCHERICHIA COLI ISOLATED FROM CLINICAL ENDOMETRITIS
2024
Rajneesh Singh | Raut Akash | Raktim Saikia | Soumen Choudhury | Ajay Pratap Singh | Amit Shukla | Jitendra Agrawal
ABSTRACT: Uterine infections in cattle and buffaloes cause severe economic loss to farmers and phytoremediation emerges as a promising alternative to synthetic antimicrobials to combat this issue. Herein, we investigated the precise mechanism of antibacterial action of Prosopis juliflora leaves (PJ) extract against a clinical isolate of E. coli with special reference to its anti-biofilm activity. PJ extract exhibited promising in vitro antibacterial action with 54.55% inhibition of bacterial biofilm production and decreased the multilayer growth of biofilms and free-living cells by influencing the integrity of cell walls as evidenced by scanning electron microscopy. Furthermore, it was observed that the damaged cell wall of the bacteria prevented the formation of clusters and prevented it from maintaining its normal morphology in the presence of the extract. Taken together, it may be inferred that PJ extract can be used to treat uterine infections as an alternative to synthetic antimicrobials; nevertheless, further investigations are warranted to ascertain its effectiveness in vivo.
显示更多 [+] 显示较少 [-]