MODULATION OF THE INFLAMMATORY CYTOKINES AND CYTOPROTECTIVE ENZYME BY BILIRUBIN TREATMENT TO ENHANCE CUTANEOUS WOUND HEALING IN RATS
2017
Raju Prasad | Dhirendra Kumar | Vinay kant | Surendra K Tandan | Dinesh Kumar
Inflammation is the main process of wound healing where expression of certain cytokines likes Interleukin-10 (IL-10) and Tumour necrosis factor ∝ (TNF ∝) plays an important role. In view of the antioxidant potential of bilirubin, the present study was aimed to evaluate time-dependent (day 3, 7, 14) wound healing effects of bilirubin ointment (0.3%) in excisional wound model in rats. Thirty-six acclimatized healthy male Wistar rats (120-150g) were divided into control and treated groups containing 18 rats each. Each group was further sub- divided into three sub-groups (day 3, 7 and 14 days, n= 6). The ointment base (soft paraffin 90%, lanolin 5% and hard paraffin 5%) and bilirubin ointment (0.3%) were applied topically once daily for 14 days in control and treated group respectively. The wound area was determined on days 3, 7, and 14. The mRNA expression of TNF ∝ gene and IL-10 gene were determined on days 3, 7 and 14 by Real Time PCR and their protein levels by ELISA method. The protein expression of cyto-protective enzyme HO-1 (Heme oxygenage-1) and growth factor VEGF (Vascular growth factor) was determined by western blotting method. The mRNA expression and protein level of TNF ∝ was significantly reduced and IL-10 was significantly increased whereas the expression of HO-1 enzyme and VEGF was significantly increased in treated group on days 3, 7 and 14. It may be concluded that the bilirubin has pro-healing potential.
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