The Prophylactic Role of Flavonoids of Black Grape (Vitis vinifera L.) on Some Biochemical Parameters in Adult females Rats Treated with Sodium Fluoride

Present study was aimed to investigate the protective role of flavonoid of black grape (Vitis vinifera L.) to contrast the damaging of 100ppm sodium fluoride(NaF) on some biochemical parameters (glucose, insulin, α-amylase and peroxy nitrate) in females rats .Twenty females rats were divided randomly into four groups and treated for (30)days as follows: control group(C) which were given tap water. First treated group (T1) was given 100ppm in drinking water,the second treated group (T2) was given flavonoids extract from black grape (300mg/kg) by oral intubation ,third group(T3) was given 100 ppm of sodium fluoride plus 300mg/kg of flavonoids of black grape .Blood samples were collected after 30 days for measuring the following parameters : glucose , insulin , α-amylase and peroxy nitrate. The results illustrated a significant increase (P˂0.05) in, blood glucose concentration of sodium fluoride treated group (T1) and sodium fluoride plus flavonoids(T3), concentration of insulin in sodium fluoride treated group ,and concentration of peroxy nitrate radical in sodium fluoride treated group (T1) and sodium fluoride plus flavonoids(T3) groups as compared with control group . Significant depression (P˂0.05) in α-amylase activity in sodium fluoride treated group (T1) and sodium fluoride plus flavonoids(T3) as compared with other treated groups was also recorded . From the autcomes of this study documented seems that treatment with flavonoids of black grape(Vitis vinifera )  at concentration of 300mg/kg B.W has the ability to correct the adverse effect of sodium fluoride in females rats.However,there is no significant differences in group treated with flavonoids as compared with control. Histological sections of liver illustrated clear impact of group treated with NaF, manifested by necrosis of hepatic cells with infiltration of inflammatory cells while animals treated with flavonoids of black grape plus NaF revealed slight necrosis area and inflammatory cells in liver. However, there are no abnormal changes in animal treated with flavonoids of black grape. In inference, the autcomes of this study documented the ameliorative effect of flavonoids of black grape apposite the noxious effect of NaF on some biochemical parameters that effect on liver function.