Bovine intranuclear coccidiosis caused by Eimeria subspherica in Oita Prefecture, Japan
2018
Kawakami, T. (Oita Prefectural Oita Livestock Hygiene Service Center, Oita (Japan)) | Matsubayashi, M. | Yamada, M. | Hitomi, T. | Sasai, K. | Shibahara, T.
An epizootiological survey using histopathological and molecular methods was conducted for bovine intranuclear coccidiosis in 199 diseased cattle at 146 farms in Oita Prefecture, Japan, from 2012 to 2016. Intranuclear coccidiosis was confirmed in nine out of 199 cattle (4.5%). They were Japanese black calves that originated from nine different farms, and showed chronic diarrhea and poor growth. Macroscopically, thickening of the small intestinal mucosa (55.6%, 5/9) and enlargement of the mesenteric lymph node (77.8%, 7/9) were observed. Histological lesions were characterized by atrophy of intestinal villi of the small intestine, inflammatory cell infiltration in the lamina propria and numerous parasites in the nucleus of epithelial cells in the small intestine. Through the molecular analyses of nine samples using 18S ribosomal RNA gene-specific primers from Eimeria species, five samples (55.6%) were successfully amplified and the parasites were found to be closely related to Eimeria subspherica. These results suggested that E. subspherica plays a significant role in bovine intranuclear coccidiosis.
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