Biosynthesis and accumulation of GABA in rice plants treated with acetic acid
2018
Isaji, S. (Kyoto University, Kyoto, Kyoto (Japan). Graduate School of Agriculture) | Yoshinaga, N. | Teraishi, M. | Ogawa, D. | Kato, E. | Okumoto, Y. | Habu, Y. | Mori, N.
Rice seedlings (Oryza sativa) that have died from drought cannot be rescued by watering afterward, but pre-treatment with exogenous acetic acid enabled the plants to produce shoots again after being watered (hereinafter referred to as “drought resilience”). To elucidate the metabolism of acetic acid, we treated rice plants with sup(13)C-labeled acetic acid and traced sup(13)C-labeled metabolites using LC-MS and sup(13)C-NMR techniques. The LC-MS and sup(13)C-NMR spectral data of the root extracts indicated that the acetic acid treatment was absorbed into the plants and then was metabolized to gamma-aminobutyric acid (GABA) by glutamic acid decarboxylase (GAD). GABA accumulation in the roots took place in advance of that in the shoots, and the survival rate against drought stress increased in proportion to the amount of GABA accumulated in the shoots. Therefore, GABA accumulation in shoots may be a key step in drought resilience induced by the acetic acid treatment.
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