Development of a programmable piggyback syringe pump and four-times-a-day injection regimen for superovulation in non-lactating Holstein cows
2015
Irshad, A.R. ((Gifu University, Gifu (Japan). United Graduate School of Veterinary Science), (Iwate University, Morioka (Japan). Faculty of Agriculture, Cooperative Department of Veterinary Medicine)) | Sasaki, T. | Kubo, T. | Odashima, N. | Katano, K. | Osawa, T. | Takahashi, T. | Izaike, Y.
The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3- < 5 mm in diameter) and large (>= 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 +- 0.4 and 3.2 +- 0.6, respectively).
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