Identification of Pseudoperonospora cubensis using real-time PCR and high resolution melting (HRM) analysis
2016
Lee, J.H. (Sejong University, Seoul (South Korea). Department of Bioresource Engineering and Plant Engineering Research Institute) | Park, M.H. | Lee, S.
To develop a molecular method to identify Pseudoperonospora cubensis in cucumber leaves with signs of downy mildew, we compared the nucleotide sequences of ribosomal DNA-internal transcribed spacer, cytochrome oxidase II, and beta-tubulin genes of P. cubensis and P. humuli isolates. Seven single nucleotide polymorphisms (SNPs) distinguished P. cubensis and P. humuli based on variations in beta-tubulin sequences, and one specific primer set was designed for further analysis. Realtime PCR and high resolution melting analysis showed that the primer set can be used to specifically identify P. cubensis in cucumber leaves with downy mildew.
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