The efficient production of dairy cattle and Wakasagyu (Japanese black) using embryo transfer (ET)
2019
Matsumoto, S. (Fukui Prefectural Livestock Experiment Station (Japan)) | Sasaki, K. | Kawamori, N.
We transferred female embryo to the opposite uterine horn of corpus luteum following to artificial insemination using sexed sperm to daily cattle (ET after AI) and surveyed the risk of it. The availability of Interferon-stimulated gene protein 15kDa (ISG15) for pregnancy diagnosis for twins was also examined. For the results, the fertility of ET after AI was 36.4% (12/33 cattle) and, was higher than the fertility of AI using sexed sperm (35.3%, 12/34 cattle) and ET (31.8%, 107/337 cattle). All the calves of singles were Holstein Friesian from AI; therefore it could be said that ET after AI could be one of effective method to improve fertility rate and product Dairy cattle. However, it had the high delivery risk due to the high rate of twins. In addition, there were the high rates of embryonic mortality and abortion, especially when cow had the non-pregnant period over 200 days. The total cholesterol of the fertilized cattle was significant higher than the unfertilized; therefore it could be said that the shortage of calorie caused the lower fertility rate. The expression level of ISG15 indicated 25.0% of twin predictive rate (twin predicted / twin pregnancy: 1/4 cattle) and it might be that this low twin predictive rate showed the determination of ISG15 could not be used for prediction of twins.
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