Germination properties of spores on entomopathogenic microsporidia, Trachipleistophora haruka isolated from Spodoptera litura
2019
Nakamura, H. (Nihon University, Fujisawa, Kanagawa (Japan). College of Bioresource Sciences, Department of Agricultural Bioscience, Laboratory of Applied Entomology) | Hatakeyama, Y. | Arai, R. | Imura, Y. | Takahashi, M. | Iwano, H.
In this paper, we report on the specific spore germination conditions of the microsporidia Trachipleistophora haruka (Old name Trachipleistophora sp. OSL-2012-10 was reported by Shigano et al. (2015)) isolated from the common cutworm, Spodoptera litura from the Ogasawara Islands, Japan. We analyzed germination rates in varying salt conditions and found that alkaline stress was required for spore germination. Nosema bombycis NIS-001 of the experiment control germinated regardless of temperature only by treatment of 0.1 M KOH or 0.1 M KCl+3% (w/v) H2O2. However, Trachipleistophora haruka treatment by 0.85% NaCl solution at 25℃ showed germination behavior similar to treatment of 0.1 M KOH and 0.1 M KCl+3% (w/v) H2O2. The results indicated that this microsporidia requires sodium or potassium ions to germinate.
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