B-cell clonality in cattle with persistent lymphocytosis caused by bovine leukemia virus infection
2020
Sakaguchi, K. (Obihiro University of Agriculture and Veterinary Medicine (Japan). Department of Veterinary Medicine, Division of Clinical Veterinary Medicine) | Maezawa, M. | Inokuma, H.
The clonality of peripheral blood B lymphocytes in cows with persistent lymphocytosis(PL)due to bovine leukemia virus infection was analyzed by using inverse polymerase chain reaction and microchip electrophoresis. As a result, a single peak was observed in all 3 cows with enzootic bovine leukemia(EBL), whereas no peaks were observed in 10 negative control animals. In 5 of 36 PL cows, on the other hand, multiple peaks were detected. These results indicate that B lymphocytes multiplied monoclonally to form tumors in EBL cattle, whereas proliferation was oligoclonal in PL cattle suggesting that lymphocytes were already tumorous before onset of EBL.
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