Genome-wide screening of genes whose enhanced expression affects glycogen accumulation in Escherichia coli
2010
Eydallin, Gustavo | Montero Macarro, Manuel | Almagro Zabalza, Goizeder | Sesma Pascual, María Teresa | Viale Bailone, Alejandro M. | Muñoz Pérez, Francisco José | Rahimpour, Mehdi | Baroja Fernández, Edurne | Pozueta Romero, Javier | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.
显示更多 [+] 显示较少 [-]This research was partially supported by the grant BIO2007-63915 from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) and by Iden Biotechnology S.L.
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