UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
2024
Palma Dovis, Leopoldo | Ruiz de Escudero Fuentemilla, Íñigo | Mañeru Oria, Francisco Javier | Berry, Colin | Caballero Murillo, Primitivo | Institute for Multidisciplinary Research in Applied Biology - IMAB
In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light.
显示更多 [+] 显示较少 [-]This research was funded by the Spanish Ministry of Science and Innovation grant number AGL2009-13340-C02 and by the Universidad Pública de Navarra. Leopoldo Palma would like to express gratitude to the Spanish Government-Universities Ministry, the NextGenerationEU and Recovery, Transformation, and Resilience plans for funding his awarded María Zambrano contract (ref. ZA21-003).
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