Non-coding stem-bulge RNAs are required for cell proliferation and embryonic development in C. elegans.
2015
Kowalski, Madzia P | Baylis, Howard A | Krude, Torsten
Stem bulge RNAs (sbRNAs) are a family of small non-coding stem-loop RNAs present in Caenorhabditis elegans and other nematodes, the function of which is unknown. Here, we report the first functional characterisation of nematode sbRNAs. We demonstrate that sbRNAs from a range of nematode species are able to reconstitute the initiation of chromosomal DNA replication in the presence of replication proteins in vitro, and that conserved nucleotide sequence motifs are essential for this function. By functionally inactivating sbRNAs with antisense morpholino oligonucleotides, we show that sbRNAs are required for S phase progression, early embryonic development and the viability of C. elegans in vivo. Thus, we demonstrate a new and essential role for sbRNAs during the early development of C. elegans. sbRNAs show limited nucleotide sequence similarity to vertebrate Y RNAs, which are also essential for the initiation of DNA replication. Our results therefore establish that the essential function of small non-coding stem-loop RNAs during DNA replication extends beyond vertebrates.
显示更多 [+] 显示较少 [-]This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC doctoral training grant DTG (BB/F016581/1) and grant BB/K013378/1).
显示更多 [+] 显示较少 [-]This is the final version of the article. It first appeared from The Company of Biologists via http://dx.doi.org/10.1242/jcs.166744.
显示更多 [+] 显示较少 [-]