Detection of coronavirus among domestic animals
2024
Sadikaliyeva, Sandugash | Shorayeva, Kamshat | Abay, Zhandos | Jekebekov, Kuanish | Shayakhmetov, Yeraly
This article presents the results of studies on biological samples collected from 640 swabs taken from dogs and cats across various regions of the Republic of Kazakhstan. These samples were part of a monitoring study on the spread of coronavirus among domestic animals. Total RNAs were isolated using the magnetic sorption method with the ALPREP kit and subsequently analysed with the ALSENSE-SARS-CoV-2 RT-qPCR kit. Real-time RT-PCR analysis revealed that 104 samples tested positive within 22–37 amplification cycles. These positive samples were then cultured in Vero cell lines to confirm the presence of the virus. The biological activity of the resulting virus-containing suspension was determined using the Reed-Muench method. During cultivation, one viral isolate with a biological activity of 5.83±0.08 lg TCID50/ml was obtained. A microphotograph of the virus was taken using an electron microscope to determine its size, shape, and structure, which confirmed its morphology corresponding to the Coronaviridae family. The data obtained further indicate that domestic animals can suffer from and carry coronavirus. It is becoming increasingly evident that the virus can infect and replicate in the organs of various farm and domestic animals.
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出版者 Latvia University of Life Sciences and Technologies
