Efectos del suero de dorada (Sparus aurata) en la composición de ácidos grasos y la respuesta inmune in vitro de macrófagos
2024
Montesinos Real, Yazmina
Seabream macrophages incubated with fetal bovine serum (FBS) and seabream serum (SS) were compared analysing fatty acid profile, phagocytic activity against Vibrio anguillarum, cell viability and respiratory burst and the gene expression of proinflammatory cytokines, related to apoptosis and antiviral activity. The results showed that macrophages likely reflect the fatty acid profile of the sera or diet. The phagocytic activity of both sera did not show statistical differences, but SS kept more macrophages alive and for longer. The basal respiratory burst resulted in greater production of reactive oxygen species (ROS) in cells treated with SS compared to FBS. The production of pro-inflammatory and anti-inflammatory cytokines and apoptotic activity was higher in FBS with LPS and poly I:C, but weaker in SS. Samples with SS reacted more with both immune stimulants, indicating that macrophages' fatty acid profile and diet may influence their immune response.
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