Assessment of Genetic Variation in Azerbaijani Hazelnut (Corylus avellana L.) Genotypes Through ISSR Markers
2024
Ilham Garagurbanl | Aytaj Asgarova | Sevda Babayeva | Elnara Ahmadova | Nargiz Abdullayeva | Vusala Izzatullayeva | Zamina Sarhadova | Sevil Suleymanova | Mehraj Abbasov
Abstract. The genetic diversity of 11 local hazelnut varieties (Corylus avellana L.) from Azerbaijan was assessed using 8 ISSR markers. A total of 48 fragments were amplified from these varieties, 15 of which were polymorphic. Among the primers, UBC 818 exhibited the highest polymorphism (55.6%), while UBC 812 showed the lowest (10%). Overal, the average polymorphism across the markers was 32%. Genetic diversity analysis revealed high diversity for UBC 818 primer, while UBC 812 displayed the lowest diversity. The mean GDI for the entire collection was 0.44. The Jaccard genetic distance index ranged from 0.14 to 0.86. The greatest value was observed between the Mirzebeyli and Galib, while the Ata-baba and Azeri, as well as the Barli and Azeri, showed the closest genetic relationships. The dendrogram analysis identified three main clusters. Principal Coordinate Analysis supported these findings, grouping the varieties into three distinct groups, with Mirzebeyli being the most genetically distinct. Additionally,molecular fingerprinting revealed unique genetic profiles for each variety, which an facilitate their identification. The use of more polymorphic primers could further enhance the accuracy and reliability of these genetic profiles. These results offer valuable insights for hazelnut breeding programs and contribute to the conservation of theselocal varieties.
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