New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust.

ALMEIDA, D. P. DE; CARNEIRO, D. G.; CAIXETA, E. T.; ALVES, D. R.; ZAMBOLIM, L.; DÊNIA PIRES DE ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; DEISY GUIMARÃES CARNEIRO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCA; DANÚBIA RODRIGUES ALVES, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA.

New and efficient AS-PCR molecular marker for selection of coffee resistant to coffee leaf rust.

2024

ALMEIDA, D. P. DE | CARNEIRO, D. G. | CAIXETA, E. T. | ALVES, D. R. | ZAMBOLIM, L. | DÊNIA PIRES DE ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; DEISY GUIMARÃES CARNEIRO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCA; DANÚBIA RODRIGUES ALVES, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA.

Coffee leaf rust, caused by Hemileia vastatrix, is the most important disease affecting Coffea arabica. The use of molecular markers through marker-assisted selection (MAS) is an effective approach to develop cultivars with multiple and durable resistance, which is economically and environmentally beneficial for this disease control. In this work, a new molecular marker based on allele-specific polymerase chain reaction (AS-PCR) associated with the quantitative trait locus (QTL) of the linkage group 5 (LG5), which confers resistance to races I, II and pathotype 001 of H. vastatrix, was developed. The CaRHv10-AS marker was validated in a segregating genetic mapping population of 247 F2 genotypes and demonstrated selection efficiency greater than 97% for genotypes with resistance to three races. Thus, this work provides a fast, robust, and affordable molecular marker for use in MAS, facilitating the development of coffee plants with multiple resistance and ensuring sustainable coffee production.

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