Integrated Metabolomics and Transcriptome Analysis of Anthocyanin Biosynthetic Pathway in <i>Prunus serrulata</i>
2025
Qi Ye | Feng Liu | Kai Feng | Tao Fu | Wen Li | Cheng Zhang | Meng Li | Zhilong Wang
<i>Prunus serrulata</i> is an important landscape tree species whose flower color has high ornamental value. However, the molecular mechanisms regulating flower color in <i>P. serrulata</i> remain unclear. By studying the metabolomics and transcriptomics of three different color varieties under the species lineage of <i>P. serrulata</i>, ‘Eigeng’ (EG, white), ‘Albo-rosea’ (AR, pink), and ‘Grandiflora’ (GF, green), the biosynthetic mechanisms of different flower colors in <i>P. serrulata</i> were revealed. The results showed that the different colors of the petals were related to the content of chlorophyll and anthocyanins. Among these, cyanidin-3-O-glucoside and cyanidin-3-O-(6-O-malonyl-β-D-glucoside) were highly expressed in AR. A combined transcriptomic analysis revealed that five flavonoid structural genes, including two <i>DFR</i> genes and three <i>UFGT</i> genes, were specifically expressed. In addition, three key transcription factors, <i>PsMYB77</i>, <i>PsMYB17</i>, and <i>PsMYB105</i>, were identified as regulators of the structural genes <i>DFR</i> and <i>UFGT</i> and participants in the forward synthesis of anthocyanin. This study provides convincing evidence elucidating the regulatory mechanisms of anthocyanin synthesis of <i>P. serrulata</i> and provides a theoretical basis for the breeding and development of new varieties and germplasm resource innovation for cherry blossom.
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