The selection of maize parent lines within marker assisted selection (MAS) by crtRB1-3'TE marker for Steppe zone of Ukraine
2023
Maize has a large genotypic diversity and a broad scale of economically important traits. Therefore, it is extremely important for breeding to obtain hybrids which can ensure high yield even under severe growing conditions, such as in Steppe zone of Ukraine. This study aimed to determine the optimal allele ratio by crtRB1-3'TE marker in parental components of maize modified hybrids. There were investigated four hundred sixteen maize hybrids which are modified hybrids of heterotic model (Iodent × Iodent) × Lancaster germplasm. SCA (specific combining ability) effects for grain yield and grain moisture content were calculated in maize hybrids with different allele combinations of crtRB1 gene. As results, the stable positive SCA effects were calculated for hybrids with allele combination (296 bp + 875 bp) × 543 bp × 296+875 bp during both 2019 and 2020 (1.23 and 0.99 t ha-1 , respectively). The lowest SCA effects for grain moisture content were obtained for modified hybrids with allele combination (543 bp × 543) × 296 + 875 bp both in 2019 and 2020 (-0.54 and -0.36%, respectively). The greatest influence SCA effects for grain yield had the interaction of allele combinations and year weather conditions (39%), the impact the allele combinations was 36%. The year weather conditions had the greatest impact on SCA effects for grain moisture content (44%), the allele combination (36%). Thus, it was determined that SCA effects for studied indicators of heterotic model (Iodent × Iodent) × Lancaster under contrasting weather condition are resulted from both genotypes of hybrid parents and favourable allele presence.
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