Effect of electrostatic interactions and complexes formation between nisin and bacterial exopolysaccharides on nisin antimicrobial efficacy
2021
Abid, Yousra | Ben Amara, Chedia | Gharsallah, Houda | Dumas, Emilie | Chihib, Nour-Eddine | Attia, Hamadi | Azabou, Samia | Gharsallaoui, Adem | Laboratoire Analyses, Valorisation et Sécurité des Aliments, Ecole Nationale d’Ingénieurs de Sfax (LAVASA) ; جامعة صفاقس - Université de Sfax - University of Sfax-المدرسة الوطنية للمهندسين بصفاقس = National Engineering School of Sfax (ENIS) ; جامعة صفاقس - Université de Sfax - University of Sfax | المدرسة الوطنية للمهندسين بصفاقس = National Engineering School of Sfax (ENIS) ; جامعة صفاقس - Université de Sfax - University of Sfax | Institut de l'Olivier | Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BIODYMIA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon | Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS) | Unité Matériaux et Transformations - UMR 8207 (UMET) ; Centrale Lille-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience
显示更多 [+] 显示较少 [-]英语. This study aims to describe interactions that may occur between nisin and bacterial exopolysaccharides (EPS) when they are present together in a model solution. For this purpose, two EPS, namely EPS-GM and EPS-BMS, produced by potential probiotic Bacillus tequilensis-GM and Leuconostoc citreum-BMS, respectively, were used. Results showed that maximal complexation occurred at pH 5.0 between anionic EPS (1.5 g/L) and cationic nisin (1 g/L) mainly through electrostatic interactions. Results provided by scanning electron microscopy, epifluorescence microscopy and turbidity measurements showed that EPS-GM was able to form bigger and higher amounts of complexes with nisin. The nisin antimicrobial activity was interestingly not affected and even improved after its complexation with EPS suggesting that these latter may act as protective agents of the nisin antimicrobial activity when applied in foods. The results of this study should help food technologists to optimize the physicochemical conditions for optimal formulation of food products containing EPS.
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