Lipid production by the oleaginous yeast <em>Yarrowia lipolytica</em> using industrial by-products under different culture conditions
2015
Rakicka, Magdalena | Lazar, Zbigniew | Dulermo, Thierry | Fickers, Patrick | Nicaud, Jean-Marc, J.-M. | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Department of Biotechnology and Food Microbiology ; Wroclaw University of Environmental and Life Sciences | Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich (ULiège) | French National Institute for Agricultural Research (INRA); INRA; French National Research Agency (Investissements d'avenir program) [ANR-11-BTBR-0003] | ANR-11-BTBR-0003,PROBIO3,Production biocatalytique de bioproduits lipidiques à partir de matières premières renouvelables et(2011) | European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012)
International audience
显示更多 [+] 显示较少 [-]英语. Background: Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. Results: In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculum densities was evaluated in fed-batch cultures. The greatest lipid content, 31% of CDW, was obtained using a low-density inoculum, a constant agitation rate of 800 rpm, and an oxygenation rate of 1.5 L/min. When the strain was cultured for 450 h in a chemostat containing a nitrogen-limited medium (dilution rate of 0.01 h(-1); 250 g/L crude glycerol), volumetric lipid productivity was 0.43 g/L/h and biomass yield was 60 g CDW/L. The coefficient of lipid yield to glycerol consumption (Y-L/gly) and the coefficient of lipid yield to biomass yield (Y-L/X) were equal to 0.1 and 0.4, respectively. Conclusions: These results indicate that lipids may be produced using renewable feedstock, thus providing a means of decreasing the cost of biodiesel production. Furthermore, using molasses for biomass production and recycling glycerol from the biodiesel industry should allow biolipids to be sustainably produced.
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