Expression of fibronectin binding protein A (FnBPA) from Staphylococcus aureus at the cell surface of Lactococcus lactis improves its immunomodulatory properties when used as protein delivery vector
2016
Almeida Barros da Silva, Juliana | Martins Breyner, Natalia | Mahi, Miloud | Ahmed, Bensoltane | Benbouziane, Bouasria | Bagano Vilas Boas, Pricilla Carolinne | Miyoshi, Anderson | Azevedo, Vasco | Langella, Philippe | Bermudez Humaran, Luis | Chatel, Jean-Marc | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil] (UFMG) | Life and Health Sciences ; Université de Djilali Bounaama Khemis Miliana (univ-DBKM) | Laboratory of Beneficial Microorganisms, Functional Foods and Health, Department of Biology ; Université Abdelhamid Ibn Badis de Mostaganem | CAPES COFECUB project [720/11]; CNPQ"Science without Border" (Brazil) ; algerian government
A recombinant strain of Lactococcus lactis displaying a cell-surface anchored fibronectin binding protein A (FnBPA) from Staphylococcus aureus (LL-FnBPA) had been shown to be more efficient in delivering plasmid than its wild-type counterpart both in vitro and in vivo, and have the ability to orientate the immune response toward a Th2 profile in a context of a DNA vaccination. The aim of this work was to test whether this LL-FnBPA strain could shape the immune response after mucosal administration in mice. For this, we used a mouse model of human papilloma virus (HPV)-induced cancer and a L. lactis strain displaying at its cell surface both HPV-16-E7 antigen (LL-E7) and FnBPA (LL-E7 + FnBPA). Our results revealed a more efficient systemic Th1 immune response with recombinant LL-E7 + FnBPA. Furthermore, mice vaccinated with LL-E7 + FnBPA were better protected when challenged with HPV-16-induced tumors. Altogether, the results suggest that FnBPA displays adjuvant properties when used in the context of mucosal delivery using L. lactis as a live vector. (C) 2016 Published by Elsevier Ltd.
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