Implication of the mineralocorticoid axis in rainbow trout osmoregulation during salinity acclimation
2011
Kiilerich, Pia | Milla, Sylvain | Sturm, Armin | Valotaire, Claudiane | Chevolleau, Sylvie, S. | Giton, Franck | Terrien, Xavier | Fiet, Jean | Fostier, Alexis | Debrauwer, Laurent | Prunet, Patrick | Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE) ; Institut National de la Recherche Agronomique (INRA) | University of Namur - Unit of Research in Organismal Biology ; Facultés Universitaires Notre Dame de la Paix (FUNDP) | Institute of Aquaculture ; University of Stirling | Xénobiotiques ; Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT) ; Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP) ; Université de Toulouse (UT)-Université de Toulouse (UT) | Université Paris-Est Créteil Val-de-Marne - Faculté de médecine (UPEC Médecine) ; Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
International audience
显示更多 [+] 显示较少 [-]英语. Cortisol and glucocorticoid receptors (GRs) play an important role in fish osmoregulation, whereas the involvement of the mineralocorticoid receptor (MR) and its putative ligand 11-deoxycorticosterone (DOC) is poorly investigated. In this study, we assessed the implication of DOC and MR in rainbow trout (Oncorhynchus mykiss) osmoregulation during hypo- and hypersaline acclimation in parallel with the cortisol-GR system. A RIA for DOC was developed to measure plasma DOC levels, and a MR-specific antibody was developed to localize MR protein in the gill, intestine, and kidney. This is the first study to report DOC plasma levels during salinity change and MR localization in fish osmoregulatory tissue. Corticosteroid receptor mRNA abundance was investigated in osmoregulatory tissue during salinity acclimation, and the effect of cortisol and DOC on ionic transporters gene expression was assayed using an in vitro gill incubation method. Differential tissue-, salinity-, and time-dependent changes in MR mRNA levels during both hyper- and hyposaline acclimations and the ubiquitous localization of MR in osmoregulatory tissue suggest a role for the MR in osmoregulation. Presumably, DOC does not act as ligand for MR in osmoregulation because there were no changes in plasma DOC levels during either freshwater-seawater (FW-SW) or SW-FW acclimation or any effect of DOC on gill ionic transporter mRNA levels in the gill. Taken together, these results suggest a role for MR, but not for DOC, in osmoregulation and confirm the importance of cortisol as a major endocrine regulator of trout osmoregulation. Journal of Endocrinology (2011) 209, 221-235
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