A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
2015
Lambert, Marine | Meudec, Emmanuelle | Verbaere, Arnaud | Mazerolles, Gerard | Wirth, Jérémie | Masson, Gilles | Cheynier, Veronique | Sommerer, Nicolas | Sciences Pour l'Oenologie (SPO) ; Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) | Centre de Recherche et d’Expérimentation sur le Vin Rosé
A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and twoadditional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies.
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