Development of cryopreservation strategies for improved reproductive competence in South Africa pig genotypes

Thesis (M. Tech. Agriculture) -- Central University of Technology, Free state, 2012

Frozen-thawed boar sperm has the potential to impact the future of the swine industry. The objectives of the study were to characterise semen of Kolbroek and Large White boars, find a suitable holding temperature and extender, determine the effect of breed and pregnancy rate following artificial insemination by frozen-thawed boar semen. A total of eight boars and 33 sows were used in this study. The 120 ejaculates were collected from each individual Kolbroek and Large White boars with the gloved-hand technique. Macroscopic and microscopic sperm characteristics were recorded. The bodyweight of Kolbroek (154.7 kg) was significantly lower compared to Large White (189.9 kg) boar. However, no significant differences were observed in Kolbroek and Large White boar semen volume (140 and 170 ml), sperm concentration (0.727 and 0.761 x 109 sperm/ml), and total sperm motility (95 and 91%). A positive correlation existed between bodyweight and semen volume of Kolbroek (r= 0.22) and Large White (r= 0.26). Conversely, the bodyweight of Large White was positively correlated to sperm concentration of Large White (r= 0.37) but negatively correlated to Kolbroek (r= -0.66). Storage time and temperature did not affect Large White boar sperm motility rate. However, Kolbroek sperm total motility rate (61.0%) was affected at 25°C after 24 hours. The highest total sperm motility rate was observed for semen diluted with Tris-based extender (74.1%) in Kolbroek boars at 48 hours of storage. Large White boar semen diluted with BTS (62.9%), Kobidil+ (69.3%) and Tris (65.1%) showed significantly higher sperm motility rate at 48 hours of storage, compared to Citrate (27.6%) extender. Cryopreservation significantly reduced sperm motility rate for Kolbroek (30.2%) and Large White (24.0%) boars. However, a high pregnancy rate was recorded in both sows of inseminated with raw diluted (100% vs. 81.3%) and frozen-thawed (50% vs. 50%) semen of Kolbroek and Large White boars. In conclusion, the bodyweight of Kolbroek and Large White boar was positively correlated with ejaculated semen volume. Kolbroek and Large White boar sperm stored at 18°C for 24 hours maintained the acceptable sperm motility rate. Kolbroek boar semen diluted with Tris-based extender maintained high sperm motility rate. Cryopreservation significantly reduced sperm motility rate regardless of breed; however, pregnancy rate from frozen-thawed semen was high for Kolbroek (50%) and Large White (50%) even though the frozen-thawed fertility was low.