Identification of two paralogous caprine CD36 genes that display highly divergent mRNA expression profiles
2013
Zidi, Ali | Castelló, Anna | Jordana, Jordi | Carrizosa, Juan | Urrutia, Baltasar | Serradilla, Juan Manuel | Amills, Marcel | Ministerio de Ciencia e Innovación (España) | Agencia Española de Cooperación Internacional para el Desarrollo | CSIC-IRTA-UAB-UB - Centre de Recerca Agrigenómica (CRAG)
The CD36 molecule plays a pivotal role in a variety of immunological and cellular processes, including pathogen recognition, inflammation and apoptosis. Herein, we demonstrate that this gene is duplicated in goats, with two copies (CD36 and CD36-like) that display highly divergent mRNA expression profiles. In this way, CD36 mRNA is mostly expressed in the adipose tissue and heart whilst CD36-like mRNA shows a high expression in the liver. We have also found evidence of the presence of two paralogous CD36 and CD36-like genes in the bovine genome, suggesting that CD36 duplication took place before goat–cattle radiation, i.e. at least 20 MYR ago. Finally, we have characterized the polymorphism of the coding regions of the goat CD36 and CD36-like genes. In doing so, we have identified one synonymous polymorphism at the CD36-like gene (c.390A>C) that displays a significant association (P = 0.04) with milk somatic cell count, a parameter often used to diagnose mastitis in domestic ruminants.
显示更多 [+] 显示较少 [-]This research was funded by projects AGL2007-66161-C02-01 and AGL2007-66161-C02-02. A. Zidi received fellowships of the Spanish Agency of International Cooperation and Development and of the Center for Research in Agricultural Genomics (CRAG).
显示更多 [+] 显示较少 [-]Peer reviewed
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