Multi-Omics Methods Applied to Flower Development
2023
Álvarez Urdiola, Raquel | Matus, José Tomás | Riechmann, José Luis | Ministerio de Economía y Competitividad (España) | European Commission | Agència de Gestió d'Ajuts Universitaris i de Recerca | Generalitat de Catalunya | Ministerio de Ciencia, Innovación y Universidades (España) | Agencia Estatal de Investigación (España)
Developmental processes in multicellular organisms depend on the proficiency of cells to orchestrate different gene expression programs. Over the past years, several studies of reproductive organ development have considered genomic analyses of transcription factors and global gene expression changes, modeling complex gene regulatory networks. Nevertheless, the dynamic view of developmental processes requires, as well, the study of the proteome in its expression, complexity, and relationship with the transcriptome. In this chapter, we describe a dual extraction method—for protein and RNA—for the characterization of genome expression at proteome level and its correlation to transcript expression data. We also present a shotgun proteomic procedure (LC-MS/MS) followed by a pipeline for the imputation of missing values in mass spectrometry results.
显示更多 [+] 显示较少 [-]Work in the authors’ laboratory was supported by grant BFU2014-58289-P (funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”) and by grant 2017SGR718 (from the Agencia de Gestió d’Ajuts Universitaris I de Recerca) to JLR, and by institutional grant SEV-2015-0533 (funded by MCIN/AEI/10.13039/501100011033) and by the CERCA Programme/Generalitat de Catalunya. R.A. is supported by fellowship PRE2018-084278 funded by MCIN/AEI/10.13039/501100011033 and by “ESF Investing in your future.” We are grateful to Eva Borràs and Eduard Sabidó from the CRG/UPF Proteomics Unit for their advice and help in proteomics research.
显示更多 [+] 显示较少 [-]Peer reviewed
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