Study of anthocyanic profiles of 123 intraspecific grape hybrids derived from Graciano x Tempranillo

Trabajo presentado en el 8th In Vino Analytica Scientia Symposium (IVAS 2013), celebrado en Reims del 2 al 5 de julio de 2013.

Berry sk:in anthocyanins are phenol compounds that are responsible for the red color ofboth grapes and wines, and are influenced by variety, climatic conditions, cultivation techniques and matmity (1]. The anthocyanic profile is used asan identification marker for grape characterization and it is under genetic control [2]. In this research, the anthocyanic profile of 123 hybrids produced by crossing two main Spanish red varieties was studied during two growing seasons (2009 and 2010) with HPLC-MS. Tempranillo and Graciano are majar wine varieties in La Rioja and are quantitatively (1.48 ± O.l3mg/g and 2.30 ± O.lOmg/g respectively) and qualitatively different in their anthocyanins content: Graciano having a higher percentage ofpeonidin (Pn) and delphinidin (Dp) monoglucosides. Fourteen monoglucosylated anthocyanins were identified. The concentration of the main five anthocyanins present in V. vinifera and the percentage of non-acylated, acetyl, and p-coumaroyl derivatives of hybrids and parents was campa red in arder to identify hybrids of enological potential interest. The mean content ofmalvidin (lvlv), delphinidin(Dp), cyaniding(Cy) and petunidin (Pt) in the 123 hybrid population showed lower values than the parents, but peonidin (Pn) content was intennediate. Although year- effect was not observed for total anthocyanins content in the two years; significan( differences were detected for all the main anthocyanins. Principal component analysis (PCA) identified two variables that explained 94.8% and 96.8% ofthe total variance present in 2009 and 2010, respectively. The first principal componen( was strongly associated with Mv-3-glu, Dp-3-glu and Pt-3-glu content and the second componen! with the Pt-3-glu content in both years. According to anthocyanic pro file, samples were divided into five groups anda group ofhybrids was identified with Pn as the majar anthocyanin. These results would be useful in the selection of new varieties with specific anthocyanic protiles.

Peer Reviewed