Modulation of cholesterol-related gene expression by dietary fiber fractions from edible mushrooms
2015
Caz, Víctor | Gil-Ramírez, Alicia | Martín-Hernández, Roberto | Marin, Francisco R. | Reglero, Guillermo | Soler-Rivas, Cristina | Comunidad de Madrid | Ministerio de Ciencia e Innovación (España)
Mushrooms are a source of dietary fiber (DF) with a cholesterol-lowering effect. However, their underlying mechanisms are poorly understood. The effect of DF-enriched fractions from three mushrooms species on cholesterol-related expression was studied in vitro. The Pleurotus ostreatus DF fraction (PDF) was used in mice models to assess its potential palliative or preventive effect against hypercholesterolemia. PDF induced a transcriptional response in Caco-2 cells, suggesting a possible cholesterol-lowering effect. In the palliative setting, PDF reduced hepatic triglyceride likely because Dgat1 was down-regulated. However, cholesterol-related biochemical data showed no changes and no relation with the observed transcriptional modulation. In the preventive setting, PDF modulated cholesterol-related genes expression in a manner similar to that of simvastatin and ezetimibe in the liver, although no changes in plasma and liver biochemical data were induced. Therefore, PDF may be useful reducing hepatic triglyceride accumulation. Because it induced a molecular response similar to hypo-cholesterolemic drugs in liver, further dose-dependent studies should be carried out.
显示更多 [+] 显示较少 [-]This research was supported by AGL2010-21537 national R+D program from the Spanish Ministry of Science and Innovation and ALIBIRD-CM S2009/AGR-1469 regional program from the Community of Madrid (Spain).
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