In vitro screening and lipid-lowering effect of prickly pear (Opuntia ficus-indica L. Mill.) fruit extracts in 3T3-L1 pre-adipocytes and mature adipocytes

Opuntia ficus-indica fruits have been widely used due to their nutritional composition and beneficial effects on health, particularly against chronic diseases such as diabetes, obesity, cardiovascular diseases and cancer, among others. In recent years, prickly pear peel and pulp extracts have been characterised, and a high number of bioactive compounds have been identified. This study aimed to analyse the triglyceride-lowering effect of prickly pear peel and pulp extracts obtained from fruits of three varieties (Pelota, Sanguinos, and Colorada) in 3T3-L1 maturing and mature adipocytes. At a concentration of 50 µg/mL, peel extracts from Colorada reduced triglyceride accumulation in pre-adipocytes and mature adipocytes. Additionally, at 25 µg/mL, Pelota peel extract decreased triglyceride content in mature adipocytes. Moreover, maturing pre-adipocytes treated with 50 and 25 µg/mL of Sanguinos pulp extract showed a reduction of triglyceride accumulation. In addition, the lipid-lowering effect of the main individual betalain and phenolic compounds standards were assayed. Piscidic acid and isorhamnetin glycoside (IG2), found in Colorada peel extract, were identified as the bioactive compounds that could contribute more notably to the triglyceride-lowering effect of the extract. Thus, the betalain and phenolic-rich extracts from Opuntia ficus indica fruits may serve as an effective tool in obesity management.

This study was supported by Ministerio de Ciencia e Innovación (PID2020-118300RB-C21 and PID2020-118300RB-C22 funded by MCIN/AEI/ https://doi.org/10.13039/501100011033 and by “ERDF A way of making Europe”, Instituto de Salud Carlos III (CIBERobn) (CB12/03/30007) and Government of the Basque Country (IT1482-22). Andrea Gómez-Maqueo acknowledges CONACyT (Mexico) for her doctoral scholarship no. 692751. Carina Proença acknowledges financial support from the project EXPL/MED-QUI/0815/2021.

Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.

Peer reviewed