Delignification of Pinus radiata kraft pulp by treatment with a yeast genetically modified to produce laccases
2010
Arana-Cuenca, A. | Téllez, Alejandro | Yagüe, S. | Fermiñán, E. | Carbajo, J. M. | Domínguez, Angel | González, Tania | Villar, J. C. | González, Aldo E. | Téllez, Alejandro [0000-0002-5491-3679]
Cellulose pulp bleaching is one of the main biotechnological applications of fungal laccases due to their capacity to degrade lignin from unbleached pulp. This application requires low cost enzyme production and higher enzyme concentrations than those obtained from the natural fungal producers. Heterologous expression of laccase in yeasts is an option for producing these enzymes on an industrial scale. In this work, we have demostrated the heterologous expression of the cglcc1 gene, responsible for laccase production in the basidiomicetous fungus Coriolopsis gallica, in the yeast Kluyveromyces lactis. In order to know if the transformed yeast has delignificant capability, a Pinus radiata kraft pulp has been incubated with it. After the treatment, a significant decrease in kappa number (13%) and in lignin content (22%) was observed. These results showed the delignificant capability of this transformed yeast. It can be concluded that the use of genetically modified microorganisms that do not demonstrate cellulolitic activity can produce high laccase levels and delignify cellulose pulps with a potential applications in cellulose pulp bleaching.
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