Feasibility of MRI and selection of adequate region of interest for longitudinal studies of growth and fatness in swine models of obesity

Barbero-Fernández, Alicia; García-Real, I.; Astiz, Susana; Ayuso, Miriam; López-Bote, C.; González De Bulnes, Antonio; Ayuso, Miriam [0000-0001-6045-394X]; Barbero-Fernández, Alicia [0000-0003-0213-6932]

Feasibility of MRI and selection of adequate region of interest for longitudinal studies of growth and fatness in swine models of obesity

2014

Purposes To determine the feasibility of MRI for imaging subcutaneous and visceral adiposity in longitudinal studies in obese swine models (Iberian pig). To establish the anatomical regions of interest (ROIs) and measurement points (MPs) adequate for their evaluation through analyses on the inter-individual variability and over-time reproducibility and through the assessment of their reliability and validity by comparison with in vivo and ex vivo zoometric data. Material and methods Five male and five female pigs were used from four (live weight around 48 kg and back-fat depth around 18 mm) to eight months old (live weight 134 kg and back-fat depth around 48 mm). MRI was carried out with a Panorama 0.23T scanner (Philips Medical Systems, Best, Netherlands), using a body/spine XL coil. Results The ROIs of election for visualization of subcutaneous data are located from the cranial margin of left diaphragmatic crura to the lumbar vertebrae L3. Visceral adiposity may be equally evaluated from the vertebrae L1 to L3. Conclusions MRI allows the evaluation of subcutaneous and visceral fatness in a single acquisition, which improves animal welfare and time- and cost-efficiency and provides an accurate, consistent and repeatable procedure for sequential studies of adiposity in obese swine. © 2014 Éditions françaises de radiologie. Published by Elsevier Masson SAS. All rights reserved.

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