Dietary probiotic supplementation shewanella putrefaciens pdp11 modulates gut microbiota and promotes growth and condition in Senegalese sole larviculture

Probiotic supplementation in fish aquaculture has significantly increased in the last decade due to its beneficial effect on fish performance. Probiotic use at early stages of fish development may contribute to better face metamorphosis and weaning stress. In the present work, we studied the influence of Shewanella putrefaciensPdp11 supplementation on growth, body composition and gut microbiota in Senegalese sole (Solea senegalensis) during larval and weaning development.S. putrefaciensPdp11 was incorporated usingArtemiaas live vector (2.5910 7 cfu mL -1 ) and supplied to sole specimens in a co-feeding regime (10–86 DAH) by triplicate. Probiotic addition promoted early metamorphosis and a significantly higher growth in length at 24 DAH larvae.S. putrefaciens Pdp11 also modulated gut microbiota and significantly increased protein content and DHA/EPA ratios in sole fry (90 DAH). This nutritional enhancement is considered especially important after weaning, where significantly higher growth in length and weight was observed in probiotic fish. Moreover, a less heterogeneous fish size in length was detected since metamorphosis till the end of weaning, being of interest for sole aquaculture production. After weaning, fish showed significantly higher growth (length and weight) and less variable lengths in fish when supplemented with probiotics. Both the enhancement of nutritional condition and the decrease in size variability associated with probiotic addition are highly interesting for sole aquaculture production.

This study was funded by Spanish National Development Plan for Sole farming (JACUMAR) and the Ministry of Science and Technology (AGL2008-05119-C02-02 and AGL 2011-30381-C03-02). The authors wish to acknowledge the assistance of the Spanish Oceanography Institute staff and especially that of Cristina Rodríguez. We are also grateful to the EULEN/FERROSER employees and especially to Javier Revilla for their assistance. Covadonga Rodríguez belongs to the Institute of Biomedical Technologies (ITB) of the Canary Islands.

Peer reviewed