Regeneration of Camellia plantlets from leaf explant cultures by embryogenesis and caulogenesis
1993
San José, M. Carmen | Viéitez Martín, Ana María
This article describes the induction of caulogenesis and somatic embryogenesis in juvenile leaf fragments of Camellia japonica and Camellia reticulata cultivar 'Mouchang'. In C. japonica cultivar 'Alba Plena' adult leaves, only callus formation occurred. Light inhibited both callus formation and morphogenesis; initial dark period of at least 14 weeks was required for bud differentiation and somatic embryogenesis. Best results were obtained using Murashige and Skoog's medium supplemented with 35.5 μM 6-benzyladenine + 2.5 μM indole-3-butyric acid. Somatic embryos and adventitious buds were successfully multiplied, and whole plantlets regenerated. For C. japonica there was a significant difference in rooting rate between shoots of axillary and adventitious origin; for C. reticulata, axillary and adventitious shoots did not differ in rooting rate, but did differ significantly in root length and the number of roots per shoot.
显示更多 [+] 显示较少 [-]Peer reviewed
显示更多 [+] 显示较少 [-]