Peptides and isoflavones in gastrointestinal digests contribute to the anti-inflammatory potential of cooked or germinated desi and kabuli chickpea (Cicer arietinum L.)
2018
Milán-Noris, Ada Keila | Gutiérrez-Uribe, Janet Alejandra | Santacruz, Arlette | Serna-Saldívar, Sergio O. | Martínez-Villaluenga, Cristina | Ministerio de Economía y Competitividad (España) | Consejo Nacional de Ciencia y Tecnología (México) | Consejo Superior de Investigaciones Científicas (España) | European Commission | Instituto Tecnológico y de Estudios Superiores de Monterrey
It is largely unknown how processing affects bioactive potential of chickpea proteins to prevent bowel inflammatory diseases. The aim was to investigate the anti-inflammatory activity of protein concentrates from germinated and cooked chickpeas (GC and CC, respectively) and its relationship with protein and isoflavone composition before and after in vitro gastrointestinal digestion and absorption. Anti-inflammatory activity of GC digests was almost 2-fold higher than CC digests (p<0.05), which was associated to greater content of peptides, formononetin and biochanin A (p<0.05). Anti-inflammatory activity of phenolic fraction in digests was 7-fold higher than the protein fraction (p<0.05). The most active peptide fraction from GC digest (IC50=93 µg/mL) contained a total of 24 peptides derived from legumin and vicilin. In conclusion, this study stands out the potential of germinated chickpea proteins concentrates to exert anti-inflammatory effects in the lower gut which may contribute to the prevention of bowel inflammatory diseases.
显示更多 [+] 显示较少 [-]A.K. Milán-Noris, acknowledges CONACYT (Consejo Nacional de Ciencia y Tecnología) for the scholarship for PhD studies (233598) and Beca Mixta. This research was supported by the Ministry of Economy and Competitiveness, Spanish National Research Council (MINECO, Spain), FEDER program (Grant Numbers AGL2013-43247-R and 201670I044), CONACYT, and NutriOmics group funds from Tecnológico de Monterrey.
显示更多 [+] 显示较少 [-]Peer Reviewed
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