RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
2023
Padilla-Roji, Isabel | Ruiz-Jiménez, Laura | Bakhat, Nisrine | Vielba-Fernández, Alejandra | Pérez-García, Alejandro | Fernández-Ortuño, Dolores | MICIN/AEI/ | “European Union NextGen erationEU/PRTR” | #NODATA# | 0000-0003-2147-358X | 0009-0009-8304-8424 | #NODATA# | 0000-0002-1065-0360 | 0000-0002-3134-3095
Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.
显示更多 [+] 显示较少 [-]This publication is part of the R+D+i projects PID2019-107464RB-C21 and PDC2021- 121373-C21, funded by MICIN/AEI//10.13039/501100011033 and by the “European Union NextGen erationEU/PRTR”. I.P.-R. was supported by a Ph.D. fellowship also from AEI, grant number PRE2020-093156.
显示更多 [+] 显示较少 [-]Peer reviewed
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