Enzymatic degradation of Elephant grass (Pennisetum purpureum) stems: Influence of the pith and bark in the total hydrolysis
2014
Pérez-Boada, Marta | Prieto Orzanco, Alicia | Prinsen, Pepijn | Forquin-Gomez, Marie-Pierre | Río Andrade, José Carlos del | Gutiérrez Suárez, Ana | Martínez, Ángel T. | Faulds, Craig B. | Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
28 p.-1 tab.-4 fig.
显示更多 [+] 显示较少 [-]The internal pith of a high energy plant, Elephant grass (EG), was more extensively degraded (>50% dry matter) compared to the outer cortex (31%) or the whole stem (35%) by an enzyme preparation from Humicola insolens, Ultraflo. Reducing sugars and acetic acid release from the pith was also higher compared to the cortex. Supplementation of Ultraflo with a type-C feruloyl esterase increased the level of deacetylation but also led to reduced solubilisation. The addition of 20% dimethylsulfoxide (DMSO) as a co-solvent also reduced the solubility of EG by Ultraflo, although acetic acid release was increased, complimenting previous results found on model substrates. The presence of DMSO was also shown to have a protective effect on xylanase activity but not acetyl esterase activity in Ultraflo. Xylan in the biomass was preferentially solubilised by DMSO, while Ultraflo removed more glucose than xylose.
显示更多 [+] 显示较少 [-]This research was supported by a Marie Curie Intra-European Fellowship to CBF, the project LIGNODECO (KBBE-3-244362) within the 7th Framework Programme of the EU, and the Spanish project LIGNOCELL (AGL2011-25379).
显示更多 [+] 显示较少 [-]Peer reviewed
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